Application

Immunocytochemmistry Analysis: A previous lot was used by an independent laboratory in IC (Van de Flier, L.G., et al., 2009)

Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in ChIP (Hatzis, P., et al., 2008).

Anti-Achaete Scute homolog 2 Antibody, clone 7E2 detects level of Achaete Scute homolog 2 & has been published & validated for use in WB, IC, ChIP.

General description

Achaete Scute homolog 2 (also known as mammalian aschaete-scute homolog 2 or Mash2) is a nuclear protein expressed specifically in the extravillous trophoblasts of developing placenta. Placental development involves control by the basic helix-loop-helix transcription factor Achaete Scute homolog 2, which may regulate HIF (hypoxia) in the formation of spongiotrophoblasts. Targeted mutagenesis of Achaete Scute homolog 2 yielded loss of function results in embryonic lethality at midgestation due to placental failure associated with a lack of spongiotrophoblasts and reduced labyrinthine trophoblast layers. In addition to healthy placental development, Achaete Scute proteins may also be involved in the determination of neuronal precursors in the central and peripheral nervous systems. Achaete Scute homolog 2 has also been implicated as a controller of intestinal stem cell fate and is essential for the maintenance of adult intestinal stem cells (Tanaka, M., et al., (1997) Dev Biol. 190(10):55-65).

Immunogen

His-tagged recombinant protein corresponding to human Achaete Scute homolog 2 near the C-terminus.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Achaete Scute homolog 2 in 10 µg of human placenta tissue lysate.

Specificity

This antibody recognizes Achaete Scute homolog 2 near the C-terminus.

Target description

~20 kDa